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Cell Line Species Identification
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| Cross-contamination and misidentification of cell lines are unfortunately very common within the research community. Many contaminated cell lines were overgrown by the contaminating cell line during establishment and so authentic stocks probably do not exist; in those cases all work has been performed on the contaminant, which may come from a different species, tissue or cell type.
Cell line cross-contamination was first discovered in the 1960s and has been extensively reported since that time. Contaminated or misidentified cell lines have also been listed on the websites of individual cell banks. To make it easier to check for these references, a single list has been developed summarising the cell lines known to be affected, their contaminants, and the references used.
Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RAF, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI (2010) Check your cultures! A list of cross-contaminated or misidentified cell lines. Int J Cancer 127 (1): 1-8. [PMID 20143388]
This table is meant as a preliminary guide to avoiding suspect cell lines, but all recently acquired cell lines should be tested (e.g. by STR profiling for human cell lines) and compared to reference stock before use. Observations made in these lists are based on published reports and details obtained from cell banks, their websites, and Wikipedia. The authors take no credit nor responsibility for any of the primary observations and have merely attempted to collate data previously available on other sites.
List of Misidentified or contaminated cell lines
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