A.704
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Description
Description: Derived from 78-year-old male. Does not produce tumours in nude mice.
Also Known As:
Species: Human
Tissue: kidney
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2×100000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: J Natl Cancer Inst 1973;51:1417
Hyperlink to ECACC Cell Line Data Sheet: 93020513
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