A7r5
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Description
Description: Flat ribbon-like cells differentiating to parallel arrays of spindle shaped cells. Derived from the thoracic aorta of an embryonic BDIX rat. As cells achieve the stationary phase there is an increase in myokinase and creatine phosphokinase (CPK) activity. After cessation of cell division a muscle type CPK isoenzyme is synthesised. If the cells are to be used in calcium channel electrophysiological studies we recommend the cells are maintained at no greater than 80% confluence.
Also Known As:
Species: Rat
Tissue: muscle
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 1-3 x10,000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C. After resuscitation cells may take up to 7 days before a monolayer forms and growth can be very slow in general. Cells also grow in clumps.
Release Conditions: No
Depositor:
Originator: No
References: Exp Cell Res 1976;98:349. Endocrinology, June 2006, 147 (6) 2675?2680
Hyperlink to ECACC Cell Line Data Sheet: 86050803
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