Description: Derived from a neck lymph node metastasis of a follicular thyroid cancer (FTC).This cell line is tumourigenic in Balb/c mice. FTC-133 (ECACC Catalogue number 94060901)”, FTC-236 (ECACC Catalogue number 06030202) and FTC-238 (ECACC Catalogue number 94060902) were all derived from the same individual, a 42 year old male with follicular thyroid cancer. This has been confirmed by short tandem repeat STR-PCR analysis at ECACC The Y chromosome could not be detected in these cell line by (STR)-PCR analysis. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell lines are identical to the source provided by the depositor based on the STR-PCR analysis.

Also Known As:

Species: Human

Tissue: Thyroid (lymph node metastasis)

Growth Properties: Adherent

Morphology: Endothelial

Growth Medium: DMEM and Ham's F12 (1:1 mixture) + 10ug/ml Insulin + 0.01U/ml Thyroid Stimulating Hormone (TSH) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C.

Release Conditions: No

Depositor:

Originator: No

References: Demeure MJ, Damsky CH, Elfman F, Goretzki PE, Wong MG, Clark OH. 1992 Invasion by cultured human follicular thyroid cancer correlates with increased beta 1 integrins and production of proteases.World J Surg. 16(4):770-6 PMID: 1384245.

Hyperlink to ECACC Cell Line Data Sheet: 06030202