Date added: 11/03/2015 Top Story: 475 Impostor Cell Lines and Counting

 

Good Cell Culture Practice

 

475 Impostor Cell Lines and Counting

 

American writer, Jill Neimark, has written a second article about the problem of cell line contamination and the researchers who are caught up in it. The article, titled "Line of attack," was published in the journal Science on 27 February 2015. Here, Neimark focuses on the work of University of Colorado geneticist, Dr Christopher Korch, and the work of the International Cell Line Authentication Committee (ICLAC), to which he belongs.  ICLAC curates a free, online database of misidentified cell lines, which now number 475.

 

To read the article, click here.

 

Click here to read Neimark's first article, which was published in the Discover magazine in November 2014.

 

To access the ICLAC database of misidentified cell lines, click here.

 

Cell-based Research

 

Additional Mechanisms of Resistance to EGFR Blockade

 

Researchers have exploited five colorectal cancer cell lines (DiFi, LIM1215, HCA-46, NCIH508, OXCO-2 and CCK81), which are highly sensitive to cetuximab, to discover additional mechanisms of resistance to EGFR blockade. Their findings were published on 26 January 2015 in a Clinical Cancer Research article titled, "Emergence of multiple EGFR extracellular mutations during cetuximab treatment in colorectal cancer." Here, the authors report the discovery of five novel point mutations in the ectodomain of EGFR, which confer resistance to cetuximab. All five cell lines used in the study were tested and resulted negative for mycoplasma contamination. The identity of each cell line was also tested and authenticated.

 

To read the article, click here.

 
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