L929/A
Mouse C3H/An", connective tissue, Adriamycin, Doxorubicin," drug resistant
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Description
Description: This Adriamycin-resistant cell line has been developed by exposure of the parent L929 murine fibroblast cell line (ECACC catalogue number 85011425) to increasing concentrations of doxorubicin in-vitro. L929/A cells can be used in the development of novel anti-cancer treatments. Resistance can be circumvented by modulating agents such as verapamil and quinine. The parent cell line L929 was derived from normal subcutaneous areolar adipose tissue.
Also Known As:
Species: Mouse
Tissue: Adipose
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: RPMI 1640 + 10% Foetal Bovine Serum (FBS) + 2mM L-Glutamine + 0.086uM Doxorubicin hydrochloride.
Subculturing Procedure: Trypsinize with 0.05% Trypsin/EDTA and inoculate new cultures vessels at 1-2 x 10e4 cells /cm2. Cultures incubated in a humidified 5% CO2/95% air incubator at 37°C.
Release Conditions: No
Depositor: Trust Diya, Staffordshire University, Science Centre, Leek Road, Stoke-on-Trent, ST4 2DE
Originator: No
References: Cadagan D Merry S. Circumvention of inherent or acquired cytotoxic drug resistance in vitro using combinations of modulating agents. Anticancer Res. 2013 Oct; 33 (10):4381-7.
Hyperlink to ECACC Cell Line Data Sheet: 14112101
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