Description: The cell line 108CC5 is formed by the fusion of mouse neuroblastoma N18TG2 and rat glioma C6-BU-1using inactivated Sendai virus. This cell line was renamed NG108-5 by Klee and Nirenberg (Proc. Natl. Acad. Sci. (1974) 71: 3474-77). This is a cholinergic hybrid cell line.

Also Known As:

Species: Mouse x rat hybrid

Tissue: neural

Growth Properties: Adherent

Morphology: Neuronal

Growth Medium: DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) HAT (0.1 mM hypoxanthine, 10 µM aminopterin and 16 µM thymidine) Cells can be cultured without HAT provided they were cultured inbetween in HT medium for 3-4 days.

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2 x 10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO2; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required.

Release Conditions: No

Depositor: Professor Bernd Hamprecht, Interfaculty Institute for Biochemistry, University of Tuebingen, Hoppe-Seyler Street 4, 72076 Tuebingen, Germany.

Originator: Yes

References: Hamprecht, (1977) Structural, electrophysiological, biochemical, and pharmacological properties of neuroblastoma-glioma cell hybrids in cell culture Int. Rev. Cytol. 49: 99-170.

Hyperlink to ECACC Cell Line Data Sheet: 08062521