Description: Transformed with sheared human Ad5 DNA. Sensitive to human adenoviruses and adenovirus DNA. Can be used to isolate transformation defective host-range mutants of Ad5 and for titrating human adenoviruses. This is a hypotriploid human cell line. The modal chromosome number was 64, occurring in 30% of cells. The rate of cells with higher ploidies was 4.2%. The der (1)t(1;15) (q42;q13), der(19)t(3;19)(q12;q13),der(12)t(8;12) (q22;p13) and four other marker chromosomes were common to most cells. Five other markers occurred in some cells only. The marker der(1) and M8 (or Xq+) were often paired. There were four copies of N17 and N22. Noticeably in addition to three copies of X chromosomes, there were paired Xq+ and a single Xp+ in most cells. The Ad insert was shown to consist of a colinear segment from nucleotides 1 to 4344 integrated into chromosome 19 (19q13.2). Expression of an unusual cell surface receptor for vitronectin has been reported. This is composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit.

PLEASE NOTE:  293 cells detach at room temperature; may take up to seven days to reattach.

Also Known As: HEK 293

Species: Human

Tissue: Kidney, embryo

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% FCS.

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. When resuscitating a frozen ampoule of cells we recommend the cells are seeded at the higher seeding level i.e. 5 x10,000/cm². Cells may take up to 7 days to attach after resuscitation and subculture. Cells detach easily at room temperature or during transit, therefore growing cultures may be received with cells in suspension. In this event, centrifuge contents of flask and reseed to allow re-attachment of cells. These cells should be frozen in 5% DMSO : 95% FCS

Release Conditions: No

Depositor:

Originator: No

References: Virology 1977 77:319 PNAS USA 1996 93:4891 PNAS USA 1996 93:4192 Virology 1978 86:10 J Biol Chem

Hyperlink to ECACC Cell Line Data Sheet: 85120602