577MF
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Description
Description: An undifferentiated carcinoma cell line established from a forehead metastasis of a malignant teratoma. This is not an embryonal carcinoma cell line. This cell line is tumourigenic in nude mice.
Also Known As:
Species: Human
Tissue: Forehead, teratoma
Growth Properties: Adherent
Morphology: Teratoma
Growth Medium: DMEM (High Glucose) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin or trypsin/EDTA; 10% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Andrews et al., 1980. A comparative study of eight cell lines derived from human testicular teratocarcinoma. Int J cancer 26:269-280, Wang et al., 1980. Nonrandom abnormalities in chromosome 1 in human testicular cancers. Cancer Res. 40:796-802
Hyperlink to ECACC Cell Line Data Sheet: 06011802
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