70Z/3

CODE: 91092002
Mouse pre-B lymphocyte

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Description

Description: Derived from a methyl nitrosourea-induced tumour in a (C57BL/6XDBA/2) F1 mouse. This line is positive for cytoplasmic Ig and negative for surface membrane Ig and TdT.

Also Known As:

Species: Mouse

Tissue: blood

Growth Properties: Suspension

Morphology: Lymphoblastoid

Growth Medium: RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Maintain cultures between 2-5×100,000 cells/ml; 5% CO2; 37°C.

Release Conditions: No

Depositor: Dr P Kincade, Oklahoma Medical Research Foundation

Originator: Yes

References: J Immunol 1978;121:641; Leukemia Res 1979;3:23; Nature 1981;292:631

Hyperlink to ECACC Cell Line Data Sheet: 91092002

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