745C2
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Description
Description: This cell line is a clone isolated from the parent line 745 TG-12. The cells are resistant to oubain and thioguanine and deficient in HGPRT. Haemoglobulin synthesis can be induced with 1.5% DMSO or 1mM oubain.
Also Known As:
Species: Mouse
Tissue: blood
Growth Properties: Suspension
Morphology: Lymphoblast
Growth Medium: Ham’s F10 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Maintain cultures between 3-9×100000 cells/ml; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Cell 1977;12:653
Hyperlink to ECACC Cell Line Data Sheet: 87030901
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