AR42J
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Description
Description: Derived from a transplantable tumour of a rat exocrine pancreas. The line is tumourigenic in nude mice, and shows significant secretion of amylase and other exocrine enzymes.
Also Known As:
Species: Rat
Tissue: pancreas
Growth Properties: Semi-adherent aggregates
Morphology: Pancreas cells
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Cells grow in hollow spheroid colonies that can attach loosely. Maintain cultures between 5000-20000 cells/cm²; 5% CO2; 37°C. Cell viability may be poor on resuscitation from frozen (approx 50%) and growth can be slow. Cells may take up to 7 days to achieve 70% confluence. Resuscitate using 20% FBS, seeding at 10000 cells/cm² and media change after 48 hours. Adherent cells should be dislodged using 0.2% EDTA. N.B. High cell viability cannot be expected during culture.
Release Conditions: No
Depositor: Dr S Watson, Department of Surgery, Queens Medical Center, Nottingham
Originator: No
References: In Vitro 1980;16:212
Hyperlink to ECACC Cell Line Data Sheet: 93100618
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