BF-11
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Description
Description: The BF-11 hybridoma cell line secretes a rat monoclonal antibody named Epoclonalan that reacts with human erythropoietin. Spleen cells from ACI strain rats that had been immunised with human urinary erythropoietin have been fused with Sp2/0 Ag-14 to produce this cell line. The alpha, beta and asialo forms of human erythropoietin are bound by the antibody to the same extent.
Also Known As:
Species: Rat/Mouse
Growth Medium: DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculturing Procedure: Maintain cultures between 100,000 – 1,0,000 cells/ml; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Proc Natl Acad Sci, USA 1982;79:5465
Hyperlink to ECACC Cell Line Data Sheet: 95050413
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