BT
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Description
Description: Derived from new-born bovine turbinate tissue. Used in propagation of BVDV and IBRV, some strains of parainfluenza 3 virus (P1-3V), some types of bovine adenovirus and enterovirus. The Bovine Viral Diarrhoea Virus (BVDV) status of this cell line was previously described as negative. Results of recent multiple molecular tests for BVDV that were undertaken by two independent testing laboratories were equivocal. This means that we cannot confirm the BVDV status of this cell line.
Also Known As:
Species: Bovine
Tissue: Turbinate
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Horse Serum (HS)
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3×10, 000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator:
References: Arch Virusforschung 1974;45:285
Hyperlink to ECACC Cell Line Data Sheet: 89031603
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