C-4 II
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Description
Description: From a cervical carcinoma of a 41 year old Caucasian female. Cells form spreading predominantly monolayered, epithelial colonies with hemicysts which are characteristic of transporting epithelia and respond to crowding by separation and shedding. These cells have retained properties of basal cell differentiation in long term culture and exhibit G6PD type B. C-4 II cells have been reported to contain human papilloma virus 18 (HPV-18) DNA sequences and to express HPV-18 RNA.
Also Known As:
Species: Human
Tissue: cervix
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: Waymouth’s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: J Nat Cancer Inst 1962;28:605; J Nat Cancer Inst 1969;43:151; J Nat Cancer Inst 1969;43:175
Hyperlink to ECACC Cell Line Data Sheet: 88102701
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