Description: A progenitor cell of the peripheral airway epithelium derived from transgenic H-2Kb-tsA58 mice capable of being passaged as an immortalised cloned cell line in culture. Cells will proliferate at 33°C in the presence of gamma-interferon, at 39°C in the absence of gamma-interferon cells will terminally differentiate. Supplied as frozen ampoules only.

Also Known As:

Species: Mouse

Tissue: Lung

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: DMEM + 2mM Glutamine + 4% Foetal Bovine Serum (FBS) + 8 supplements: 0.25 ?g/ml Endothelin-1; 0.01 ?g/ml Interferon gamma; 10 ?g/ml Insulin; 5 ?g/ml Transferrin; 7.5 ?g/ml Endothelial Cell Growth Supplement (ECGS); 0.025 ?g/ml Epidermal Growth Factor; 0.36 ?g/ml Hydrocortisone; 0.02 ?g/ml T3. For guidance on the supplements and preparation of the culture medium please refer to the product document: T7 and C22(Clara) Culture Medium.pdf.

Subculturing Procedure: Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4×10,000 cells/cm² using trypsin/EDTA; 10% CO2; 33°C. Doubling time 48h

Release Conditions: No

Depositor: Dr Daphne deMello, Department of Pathology, Phoenix Childrens Hospital, 1919 East Thomas Road, Phoenix, Arizona 85016, USA

Originator: Yes

References: Demello et al.,” (2002) Generation and characterization of a conditionally immortalized lung Clara cell line from the H-2K In Vitro Cell. Dev. Biol.-Animal 38: 154-164

Hyperlink to ECACC Cell Line Data Sheet: 07021401