C2C12
[Create a profile and sign in to view pricing and add to cart]
- Description
- Ask a Question
Description
Description: Subclone from myoblast line established from normal adult C3H mouse leg muscle. Differentiates rapidly; produces extensive contracting myotubes expressing characteristic muscle proteins. Provides model to study in vitro myogenesis and cell differentiation.
Also Known As:
Species: Mouse
Tissue: muscle
Growth Properties: Adherent
Morphology: Myoblast
Growth Medium: DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum (FBS).
Subculturing Procedure: Cells can be relatively slow growing when resuscitated from frozen, taking 4-5 days to reach 50% confluence when seeded at 2×1, 000 cells/cm². Split semi-confluent cultures 1:3 to 1:6 i.e. seeding at 1-2×1,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Do not allow cells to reach confluence.
Release Conditions: No
Depositor: Obtained from ATCC
Originator: No
References: Nature 1977;270:725, Science 1985;230;758-766, J. Cell Biol. 1994; 127:1755-1766[published erratum appears in J Cell Biol 1995 Feb;128(4):following 713], J.Virol. 1997;71:169-178, Proc. Natl. Acad.Sci. USA 1996;93:14082-14087, J. Biol. Chem. 1996;271:1386
Hyperlink to ECACC Cell Line Data Sheet: 91031101
Ask a Question
Have a question about this product? Our technical team is here to help.
Related products
-
1.2B4
CODE: 10070103A human pancreatic Beta cell: HuP-T3 hybrid cell line. Insulin-secreting cell line. -
-
