C6
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Description
Description: Derived from a rat glial tumour induced by N-nitrosomethylurea. The cells produce S-100 protein which is found in the neural tissue of vertebrates and has been found in brain tumours. Synthesis of S-100 increases 10 fold as cells reach confluency.
Also Known As:
Species: Rat
Tissue: Neural (glial tumour)
Growth Properties: Adherent
Morphology: Neuronal
Growth Medium: Ham’s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3×10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Science 1968; 161:370
Hyperlink to ECACC Cell Line Data Sheet: 92090409
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