Description: Established from the lung tissue of a 2.5 month old female with cerebral anoxia, cardiac anomaly, sepsis, endocardial cushion defect and foetal alcoholic syndrome. The cells can undergo up to 19 population doublings. CCD 18Lu (90112706) and CCD-18Co (90070503) were derived from the lung and colon respectively from the same patient; this has been confirmed by STR profiling at ECACC. Ethnicity: Black.

Also Known As:

Species: Human

Tissue: lung

Growth Properties: Adherent

Morphology: Fibroblast

Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 1-3×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Following resuscitation this cell line is initially slow to start.

Release Conditions: No

Depositor: Obtained from ATCC

Originator: No

References: Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PMID:3933111. Hinterleitner TA, et al. Il-1 stimulates intestinal myofibroblast COX gene expression and augments activation of CI- secretion in T84 cells. Am. J. Physiol. 271: C1262-C1268, 1996. PMID:8897833

Hyperlink to ECACC Cell Line Data Sheet: 90112706