COLO 206F
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Description
Description: COLO 206F, COLO 201 (ECACC Catalogue 87091201) and COLO 205 (ECACC Catalogue 87061208) were all established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). The cell line can be used for immunological studies in cytotoxicity assays and immunoglobin characterisation. Please note this cell line was mycoplasma eradicated at ECACC.
Also Known As:
Species: Human
Tissue: Colon
Growth Properties: Semi-adherent
Morphology: Predominantly round, refractile
Growth Medium: RPM11640, 10% Foetal Bovine Serum (FBS) + 2mM Glutamine.
Subculturing Procedure: Maintain cultures between 3-9×100,000 cells/ml 5% CO2, 37°C. Any attached cells can be dislodged with trypsin/EDTA.
Release Conditions: No
Depositor: Prof G Moore, Colorado Oncology Foundation, Denver General Hospital, 777 Bannock Street, Denver, Colorado. 80204-4507
Originator: Yes
References: Semple TU, Quinn LA, Woods LK, Moore GE 1978.Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38(5):1345-55 PMID: 565251.
Hyperlink to ECACC Cell Line Data Sheet: 93052620
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