COLO 839

CODE: 93051119
Human Caucasian B lymphocyte

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Description

Description: Derived from the peripheral blood of a 43 year old Caucasian male with widespread melanoma. The cells were transformed with defective EBV (B-95-W8). COLO 839 (ECACC Catalogue number 93051119)”, COLO 858 (ECACC Catalogue number 93052613) and COLO 857 (ECACC Catalogue number 93051120) were all derived from the same 43 year-old male patient with malignant melanoma (see references below). This has been confirmed by STR profiling.

Also Known As:

Species: Human

Tissue: blood

Growth Properties: Suspension

Morphology: Lymphoblastoid

Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Maintain cultures between 2-9×100,000 cells/ml; 5% CO2; 37°C.

Release Conditions: No

Depositor: Prof G Moore, Colorado Oncology Foundation, Denver General Hospital, Co 80204-4507

Originator: Yes

References: Morse HG, Moore GE (1993) Cytogenetic homogeneity in eight independent sites in a case of malignant melanoma Cancer Genet Cytogenet. 69(2):108-12. PMID: 8402545.

Hyperlink to ECACC Cell Line Data Sheet: 93051119

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