Description: Derived from a right axial lymph node of a 43 year old male with melanoma. COLO 839 (ECACC Catalogue number 93051119)”, COLO 858 (ECACC Catalogue number 93052613) and COLO 857 (ECACC Catalogue number 93051120) were all derived from the same 43 year-old male patient with malignant melanoma (see references below). This has been confirmed by STR profiling. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Also Known As:

Species: Human

Tissue: skin

Growth Properties: Adherent

Morphology: Fibroblast

Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin/EDTA; 37°C; 5% CO2.

Release Conditions: No

Depositor: Prof G Moore, Colorado Oncology Foundation, Denver General Hospital, Co 80204-4507

Originator: Yes

References: Morse HG, Moore GE (1993) Cytogenetic homogeneity in eight independent sites in a case of malignant melanoma Cancer Genet Cytogenet. 69(2):108-12. PMID: 8402545.

Hyperlink to ECACC Cell Line Data Sheet: 93052613