CP5
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Description
Description: A bovine (Bos taurus, Holstein-Fresian) articular cartilage progenitor cell line able to re-differentiate into articular chondrocytes after extensive population doublings (>30pd).
Also Known As:
Species: Bovine
Tissue: Articular cartilage
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 50µg/ml ascorbate + 10mM HEPES
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:8 using 0.25% trypsin or trypsin/EDTA; 5% CO2;37°C.
Release Conditions: No
Depositor: Professor Charles Archer, Connnective Tissue Biology Labs, Cardiff School of Biosciences, Museum Avenue, Cardiff, CF10 3US, Wales, UK
Originator: Yes
References: Dowthwaite et al., (2004) The surface of articular cartilage contains a progenitor cell population. J Cell Sci, Feb 29:117 (Pt6) 889-97. PMID: 14762107
Hyperlink to ECACC Cell Line Data Sheet: 08052101
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