CRI-G1
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Description
Description: Established from a NEDH rat transplantable islet cell tumour. The cells secrete insulin and glucagon.
Also Known As:
Species: Rat
Tissue: Pancreas
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. The cells tend to grow in clumps and ribbons. They do not strictly attach but stick lightly and will not form a complete monolayer.
Release Conditions: Yes
Depositor: Prof C N Hales, Department of Clinical Biochemistry, University of Cambridge
Originator: Yes
References: J Endocrinol 1986;109:193
Hyperlink to ECACC Cell Line Data Sheet: 87052701
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