CRI-G5
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Description
Description: Established from a NEDH rat transplantable islet cell tumour. The cells secrete insulin and glucagon. This cell line can be used in place of CRI-D11 (ECACC catalogue no. 88031604).
Also Known As:
Species: Rat
Tissue: pancreas
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 10% CO2; 37°C. The cells tend to grow in clumps and ribbons.
Release Conditions: No
Depositor: Prof C N Hales, Department of Clinical Biochemistry, University of Cambridge
Originator: Yes
References: J Endocrinol 1986;109:193
Hyperlink to ECACC Cell Line Data Sheet: 88031603
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