Description: CTLL is a subclone of T cells derived from a C57bl/6 mouse. The cells require IL-2 for growth and are used to assay for its presence in conditioned media.

Also Known As:

Species: Mouse

Tissue: Blood

Growth Properties: Suspension

Morphology: Lymphoblast

Growth Medium: RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human Recombinant IL-2 + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 4-5 x 100,000 cells/ml. Viability may be poor on resuscitation and may decrease further within the first 48 hours, the culture may appear non-viable at this time. Clusters of cells will eventually appear but this could take 1-2 weeks, during this time large media changes are not recommended. It may take 2-3 weeks for vigorous growth to be established. At subculture perform a cell count and maintain cultures between 3-9 x 100,000 cells/ml, 5% CO2, 37°c. For cryopreservation of CTLL-2, freeze at approximately 6 x 1,0,000 cells/ml in 90% FBS + 10% DMSO.

Release Conditions: No

Depositor:

Originator: No

References: Gillis S, Smith KA. 1977 Long term culture of tumour-specific cytotoxic T cells. Nature 268:154-6 PMID: 145543

Hyperlink to ECACC Cell Line Data Sheet: 93042610