Detroit 539
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Description
Description: Derived from a female caucasian infant with Down Syndrome. Karyotypic analysis shows 90% of the cell population possesses 47 chromosomes with 5 chromosomes belonging to G group. These findings persist essentially unchanged throughout at least 30 serial subcultures.
Also Known As:
Species: Human
Tissue: skin
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 3-5×10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. A further 15-20 passages expected.
Release Conditions: No
Depositor:
Originator: No
References: None specified by depositor
Hyperlink to ECACC Cell Line Data Sheet: 91031102
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