Description: Established from a lymphoma induced in a C57BL/6N mouse by 9,10-dimethyl-1,2-benzanthracene. The cells have been reported to produce low levels of type G (Gross) virus antigen (J Nat Cancer Inst 1972;48:265) H-2b, IL-4 and Thy-1.2 antigen. The cell line is resistant to cortisol and dexamethasone and is sensitive to PHA.

Also Known As:

Species: Mouse

Tissue: blood

Growth Properties: Suspension

Morphology: Lymphoblast

Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: When starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100 ul, to count cells. Centrifuge the cell suspension at low speed i.e. 100 – 150 x g for a maximum of 5 minutes. Remove medium and re-suspend the cell pellet at a density of 3 – 5 x 100,000 cells/ml in fresh medium. Incubate flask at 37°C; 5 – 7% CO2. Check daily. Subculture once exponential growth is established and maintain cultures between 3 – 9 x 100, 000 cells/ml.

Release Conditions: No

Depositor:

Originator: No

References: Br J Cancer 1950;4:372; Cancer Res 1965;25:813; J Immunol 1972;108:1146; J Nat Cancer Inst 1972;48:265

Hyperlink to ECACC Cell Line Data Sheet: 85023105