EMT6/CPR
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Description
Description: The cisplatin-resistant variant of the mouse mammary tumour cell line EMT6/P (ECACC catalogue no. 96042344) was derived by continuous in vitro exposure to increasing concentrations of cisplatin over a period of 6 weeks. EMT6/CPR was shown to express 2.5-fold resistance to cisplatin as compared with the sensitive parent line. Development of drug resistance was accompanied by a 1.7-fold increase in cellular glutathione (GSH). Growth for 4 weeks in the absence of cisplatin was reported to lead to loss of resistance phenotype and elevated GSH levels in the resistant cell line EMT6/CPR.
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Species: Mouse
Tissue: Unknown
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: MEM(EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 0.25µg/ml, cisplatin + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. It is recommended to culture the cells without drugs upon resuscitation until the first passage.
Release Conditions: No
Depositor: Dr P Twentyman, Dr. K. Wright, UKCCR, PO Box 123, Lincolns Inn Fields, London WC2A 3PX
Originator: Yes
References: Cancer Chemother Pharmacol 1991;28:273
Hyperlink to ECACC Cell Line Data Sheet: 96042318
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