EPC
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Description
Description: This cell line known as Epithelioma papulosum cyprini (EPC) was originally reported to be from Carp (Cyprinus carpio) epidermal herpes virus-induced hyperplastic lesions. More recently EPC was found to be derived from Fathead Minnow (Pimephales promelas) see Winton et al.”, 2010. This has been confirmed by DNA sequencing at ECACC. The cells support the growth of pancreatic necrosis virus and rhabdovirus.
Also Known As:
Species: Fish
Tissue: Skin
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin/EDTA; 5% CO2; 20°C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before shipping.
Release Conditions: No
Depositor:
Originator: No
References: Fijan N et al., 1983 Some properties of the epithelioma papulosum cyprini (EPC) cell line from Carp Cyprinus carpio. Ann. Virol. (Inst. Pasteur) 134E 207-220.
Hyperlink to ECACC Cell Line Data Sheet: 93120820
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