FL (HeLa derivative)
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Description
Description: Originally derived from normal amniotic tissue. Have been used and studied extensively including virus susceptibility, tumorigenicity and morphology. Contains HeLa marker chromosomes and exhibits type A G6PD. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. The cells are capable of growth in a variety of media supplemented with sera from several species. Ethnicity: Black.
Also Known As:
Species: Human
Tissue: Cervix
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: EMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4×10, 000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Cancer Res 1958;18:692; J Nat Cancer Inst 1959;23:893; Proc Soc Exp Biol Med 1965;119:223; Arch Ges Virus forsch 1960;9:559
Hyperlink to ECACC Cell Line Data Sheet: 90111910
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