Description: FM-55-p.R22.2 (ESTDAB-015) was established from a primary malignant melanoma of a male patient. The FM-55-p.R22.2 cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commission's fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB (http://www.ebi.ac.uk/ipd/estdab) contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. This information is appended below. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation. Robinson J, Waller MJ, Fail SC, Marsh SG. 2006 The IMGT/HLA and IPD databases. Hum Mutat 27(12):11929.PMID: 16944494. Pawelec G, Marsh SG. 2006 ESTDAB: a collection of immunologically characterised melanoma cell lines and searchable databank. Cancer. Immunol Immunother. 55(6):623-7.PMID: 16421722. Robinson J, Roberts CH, Dodi IA, Madrigal JA, Pawelec G, Wedel L, Marsh SG.2009 The European searchable tumour line database. Cancer. Immunol Immunother. 58(9):1501-6. PMID: 19172270. ECACC also has available two other cell lines established from metastatic tumours from the same patient (FM-55-M1 ECACC Catalogue no 13012546 and FM-55-M2 ECACC Catalogue no 13012559) and the primary tumour (FM-55-P ECACC Catalogue no 13012417).

Also Known As:

Species: Human

Tissue: Skin

Growth Properties: Adherent

Morphology: Fibroblastic

Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) seed new cultures at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density approx. 20 x 10,000 cells/cm².

Release Conditions: No

Depositor: J Zeuthen, Department of Tumour Cell Biology, Division of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, Bldg 7.1, DK-2100 Copenhagen Denmark.

Originator: Yes

References: Kirkin AF, Petersen TR, Olsen AC, Li L, thor Straten P, Zeuthen J.1995 Generation of human-melanoma-specific T lymphocyte clones defining novel cytolytic targets with panels of newly established melanoma cell lines. Cancer Immunol Immunother. 41(2):71-81. PMID: 7656272.

Hyperlink to ECACC Cell Line Data Sheet: 13012462