h1RPE7
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Description
Description: The h1RPE7 cell line was derived from primary human retinal pigment epithelial cells obtained from a 50-year-old female Caucasian donor. The primary culture was transfected with a construct encoding SV40 large T antigen that was deleted for the small t antigen along with puromycin resistance gene.
Also Known As:
Species: Human
Tissue: Eye
Growth Properties: Adherent
Morphology: Polygonal
Growth Medium: Hams F10 + 20% Heat-inactivated FCS + 2mM Glutamine + selection antibiotic 1µg/ml puromycin.
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 24hrs.
Release Conditions: Yes – commercial organisations are required to complete the ‘Cell Line Release Authorisation for Research Use in Commercial Organisations’ release conditions form.
Depositor: Professor John Greenwood, University College London Institute of Opthalmology, University College London, Bath Street London ECIV 9EL.
Originator: Yes
References: Lund et al.,” (2001) Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats. Proc. Natl. Acad. Sci. 98: 9942-9947 PMID: 11504951.
Hyperlink to ECACC Cell Line Data Sheet: 09061602
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