H4II
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Description
Description: From H-35 Reuber hepatoma induced by chemical carcinogenesis in an AxC rat (see Nat Cancer Inst Monogr 1964;13:229). Tyrosine aminotransferase positive. Synthesises several serum proteins including albumin, transferrin and prothrombin. (Same as H4-11-E-C3 (ECACC catalogue no. 85061112)).
Also Known As:
Species: Rat
Tissue: skin
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: Coon’s modified Ham’s F12 (or Kaign’s modified Ham’s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split semi-confluent [60%-70%] cultures 1:100 i.e. seeding at 2×100 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density is reached at 15×1000,000 cells/10cm² dish.
Release Conditions: No
Depositor: Dr M Weiss, Dept. Biologie Moleculaire, Institute Pasteur, Paris
Originator: Yes
References: Nat Cancer Inst Monogr 1964;13:229; J Nat Cancer Inst 1961;26:891.
Hyperlink to ECACC Cell Line Data Sheet: 89042702
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