Description: H5 is a subclone of H4-II-E-C3 (ECACC catalogue no. 85061112) which originated from the Reuber H-35 hepatoma induced by chemical carcinogenesis in an A x C rat. The dedifferentiated H5 cells express high levels of glucose-6-phosphate dehydrogenase and exhibit a population doubling time of 12 hours. Differentiated variants are H4IIEC3 (85061112)”, Fao and others (see Biochimie 1974;56:1603 for details).

Also Known As:

Species: Rat

Tissue: hepatoma

Growth Properties: Adherent

Morphology: Fusiform

Growth Medium: Coon’s modified Ham’s F12 (or Kaign’s modified Ham’s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:50 to 1:100 i.e. seeding at 1-2×1,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: No

Depositor: Dr M Weiss, Dept. Biologie Moleculaire, Institute Pasteur, Paris

Originator: Yes

References: Biochimie 1974;56:1603; Cell Growth and Differentiation 1994;5:957

Hyperlink to ECACC Cell Line Data Sheet: 94101905