HCA-2
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Description
Description: Established from a sigmoid colon adenocarcinoma of a 83 year-old female patient. The adenocarcinoma was well differentiated and determined to be Dukes' stage C. Imperial College Innovations has stated that this cell line is not to be supplied to commercial organisations.
Also Known As:
Species: Human
Tissue: Colon
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Subculturing Procedure: Split subconfluent cultures (70-80%) 1:2 to 1:10 using 0.25% trypsin/EDTA; 7.5% CO2; 37°C. Suggested seeding density 2-4×10,000 cells/cm².
Release Conditions: Yes – commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form.
Depositor: Dr S Kirkland, Department of Histopathology, Imperial College, London, Hammersmith Campus, DuCane Road, London, W12 0NN
Originator: Yes
References: S. C. Kirkland and I. G. Bailey. (1986) Establishment and characterisation of six human colorectal adenocarcinoma cell lines. Br J Cancer,53,6, 779-85.
Hyperlink to ECACC Cell Line Data Sheet: 06061901
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