HCA-7 Colony 26
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Description
Description: Subpopulation isolated from the HCA-7 cell line
Also Known As:
Species: Human
Tissue: colon
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM (High glucose Sigma D6546) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:5 to 1:10 weekly using 0.0125% trypsin/EDTA; 7.5% CO2; 37°C. Suggested seeding density 2-4×10,000 cells/cm².
Release Conditions: Yes – commercial organisations are required to complete the ‘Cell Line Release Authorisation for Research Use in Commercial Organisations’ release conditions form.
Depositor: Dr S Kirkland, Department of Histopathology, Imperial College London, Hammersmith Campus, DuCane Road London W12 0NN
Originator: Yes
References: Kirkland & Bailey 1986 Establishment and characterisation of six human colorectal adenocarcinoma cell lines. Br J Cancer 53: 779-785. PMID: 3718830
Hyperlink to ECACC Cell Line Data Sheet: 09071511
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