Description: The human colon cancer cell line, HCA-7 Colony 29 expresses Cox-2. An interesting feature of this cell line is that it forms polarised epithelial sheets which allows examination of the trafficking and release of biomolecules in vitro. Cyclooxygenases (COXs) are key enzymes in the conversion of arachidonic acid to prostaglandins and other eicosanoids. It has been found that colonic polyps and cancers overexpress Cox-2 and that the inhibition of this enzyme by nonsteroidal anti-inflammatory drugs decreases the risk of colonic neoplasia. This cell line is useful for studying the role of Cox-2 in cancer cell biology and the investigation of colorectal epithelial cell polarity. Imperial College Innovations has stated that this cell line is not to be supplied to commercial organisations.

Also Known As:

Species: Human

Tissue: Colon

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: DMEM + 10% FBS + 2mM L-Glutamine + 110mg/L Sodium Pyruvate (NaP).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2×10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: Yes – commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form.

Depositor: Dr S C Kirkland, Dept. Histopathology, Faculty of Medicine, Imperial College, Hammersmith Campus, DuCane Raod, London. W12 ONN.

Originator: Yes

References: Mann et al. (2001) Gasteroenterology 120 1713-1719, Coffey et al. (1997) PNAS 94 657-662, Marsh et al. (1993) J. Pathology 170 441-450, Kirkland (1985) Cancer Research 58 323-2327

Hyperlink to ECACC Cell Line Data Sheet: 02091238