Hepa-1c1c7
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Description
Description: Hepa-1c1c7 was cloned from the cell line Hepa-1cl which was obtained from Hepa-1. Hepa-1 was derived from the BW7756 hepatoma that developed in a C57L mouse. Hepa-1c1c7 cells express the aryl hydrocarbon (Ah) receptor and were shown to be highly inducible for cytochrome P450IA1. A variety of mutants from this cell line have been established.
Also Known As:
Species: Mouse
Tissue: hepatoma
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: Alpha MEM without nucleosides + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Proc Natl Acad Sci, USA 1979;76:373; Biochemie 1991;73:61
Hyperlink to ECACC Cell Line Data Sheet: 95090613
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