Description: The HRA-19 cell line was established from a primary human rectal adenocarcinoma present in a 66-year old male. The adenocarcinoma was well differentiated and determined to be Dukes' stage B. The HRA-19a1.1 cell line is a clone of HRA-19. HRA-19 has stem cell properties and undergoes a multilineage differentiation in serum free medium.

Also Known As:

Species: Human

Tissue: Rectum

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: DMEM (High glucose Sigma D6546) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:5 to 1:6 weekly using 0.0125% trypsin/EDTA; 7.5% CO2; 37°C. Suggested seeding density 2-4×10,000 cells/cm².

Release Conditions: Yes – commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form.

Depositor: Dr S Kirkland, Department of Histopathology, Imperial College London, Hammersmith Campus, DuCane Road London W12 0NN

Originator: Yes

References: Kirkland & Bailey 1986 Establishment and characterisation of six human colorectal adenocarcinoma cell lines. Br J Cancer 53: 779-785. PMID: 3718830. Kirkland 1986 Endocrine differentiation by a human rectal adenocarcinoma cell line (HRA-19). Differentiation 33: 148-155. PMID: 3569697

Hyperlink to ECACC Cell Line Data Sheet: 09071517