Description: The cell line HSG was originally thought to be established from a human submandibular gland from a 54 year old Japanese male. The cell line has since been found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

Also Known As:

Species: Human

Tissue: Cervix

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: MEM +2mM Glutamine +1% Non Essential Amino Acids (NEAA) + 10% New Born Calf Serum (NBCS).

Subculturing Procedure: Split confluent cultures 1:4 to 1:10 ie seeding at 1-3×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2, 37°C

Release Conditions: No

Depositor:

Originator: No

References: Cancer 1981;48:745; Cancer 1984;54:2959; Cancer 1985;55:2575; Cancer Res 1985;45:6160

Hyperlink to ECACC Cell Line Data Sheet: 95031024