HT-STAR-A
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Description
Description: HT1080 cells serially transfected with synthetic HIV Gag-Pol sequences expressed from a Murine Leukaemia Virus (MLV) vector; HIV tat sequences expressed from a MLV vector; HIV rev sequence expressed from a MLV vector and amphotropic envelope protein from a MLV vector. These cells stably express synthetic HIV Gag-Pol, HIV tat, HIV rev sequences and amphotropic envelope protein. These cells serve as a packaging cell line for HIV based vectors.
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Species: Human
Tissue: Fibrosarcoma
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 3-5×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 10% CO2; 37°C.
Release Conditions: Yes. All customers are required to complete a Material Transfer Agreement
Depositor: Professor Mary Collins, Department of Immunology and Molecular Pathology, University College London
Originator: Yes
References: Ikeda Y et. al., Nat. Biotechnol. (2003) May: 21(5): 569-572
Hyperlink to ECACC Cell Line Data Sheet: 04072123
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