Description: Cells possess Ca antigen as defined by the Ca1 antibody. The Ca antigen has been shown to be absent in non-malignant human cell strains and is considered to be a marker linked with malignancy. Island cell growth will degenerate if left more than 9 days between passages.

Also Known As:

Species: Human

Tissue: Colon

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells grow relatively slowly, in islands.

Release Conditions: No

Depositor:

Originator: No

References: Lancet 1982;2:1

Hyperlink to ECACC Cell Line Data Sheet: 85061104