II b
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Description
Description: The hybrid cell line II b has been established by transforming mouse NIH 3T3 (ECACC catalogue no. 93061524) with mitotic chromosomes from the human cell line MNNG-HOS (ECACC catalogue no. 87070201). Various probes showed stable integration of chromosome 7 DNA (7q22-31.3 and 7q22-7 cen) being closely linked to the gene mutation causing cystic fibrosis
Also Known As:
Species: Human/mouse hybrid
Tissue: Unknown
Growth Properties: Adherent
Morphology: Fibroblast-like
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 using trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Nucleic Acids Res 1986;14:7159
Hyperlink to ECACC Cell Line Data Sheet: 86072805
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