Date added: 30/11/2016 40 Funded JCRB-supplied Cell Line Vials for Australian Non Communicable Disease Research
THE CELLBANK AUSTRALIA NEWSLETTER
The Australian Government, through the Australia-Japan Foundation (AJF; Canberra, AU) of the Department of Foreign Affairs and Trade, has provided funding support that will enable CellBank Australia to provide 40 Japan-derived cell line vials to up to 40 Australia-based researchers, ** free-of-charge **, for use in Non Communicable Disease (NCD) research. All 40 vials will be supplied from CellBank Australia's catalog of JCRB Cell Bank cell lines. Scroll down the page to learn more.
Unraveling the Molecular Pathogenesis of Several Neurodegenerative Diseases
Researchers have created a panel of mammalian cell lines expressing a fragment of tau fused to yellow fluorescent protein and have demonstrated that tau prions from patients with tauopathies, including Alzheimer’s disease and chronic traumatic encephalopathy, can infect mammalian cells. Their article, titled "Tau prions from Alzheimer’s disease and chronic traumatic encephalopathy patients propagate in cultured cells," was published online in the journal, PNAS, on 28 November 2016. HEK293T cells were used in the study.
To access the article, click here.
Good Cell Culture Practice
What are the Odds of Obtaining a False Cell Line?
A study of 848 leukemia-lymphoma cell lines received by the Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ; Braunschweig, DE) from 1990 to 2014, has found that over those 25 years the incidence of mycoplasma-contamination decreased significantly, while cross-contamination of cell lines remained at an unacceptable high level, particularly among cell lines circulating unchecked between laboratories. In their 21 November 2016 International Journal of Cancer article, "False and Mycoplasma-Contaminated Leukemia-Lymphoma Cell Lines – Time for a Reappraisal," Drs Drexler, Dirks, MacLeod and Uphoff of the DSMZ said their data indicated that: "using non-authenticated cell lines from secondary sources carries a risk of about 1:6 for obtaining a false cell line."
To read the article, click here.
Incorrect Strain information for Mouse Cell Lines
A study of 80 cell lines registered with the JCRB Cell Bank (JCRB; Osaka, JP) has revealed by simple sequence length polymorphism analysis and comparisons with 7 major inbred strains that 12 of the cell lines (15%) differed from registered information. Their article, titled "Incorrect strain information for mouse cell lines: sequential influence of misidentification on sublines," was published in the journal, In Vitro Cellular & Developmental Biology - Animal, on 14 November 2016.
To read the article, click here.
ECACC November 2016 Newsletter
Click here to read about cancer drug resistance in A2780 ovarian cancer cell lines in the European Collection of Authenticated Cell Cultures (ECACC; Salisbury, UK) November 2016 newsletter.
How CellBank Australia can Help
Mycoplasma can have major impacts on cell culture results without any visible signs of contamination, and have long been recognized as common cell culture contaminants. CellBank Australia provides a two-assay mycoplasma testing service for ** $126 Australian dollars ** per sample, excluding GST.
To learn more about our service, click here.
Human Cell Authentication Testing
For human cells, CellBank Australia uses Short Tandem Repeat profiling to examine the number of tetranucleotide repeats at various loci to differentiate cell lines from different donors. CellBank Australia now offers human cell authentication testing for just ** $166 Australian dollars ** per sample, excluding GST.
For more information about our service, click here.
Japan-derived Cell Lines for Australia-based Preclinical NCD Research
Non Communicable Diseases (NCDs), mainly cardiovascular diseases, cancers, chronic respiratory diseases and diabetes, kill more people each year than all other causes combined. According to the World Health Organisation, while NCDs were responsible for 68% of global mortality in 2012, the mortality rate was 80% in Japan and 90% in Australia. Thus NCDs are a mutual concern for both nations.
To mark the Australia-Japan Foundation 40th anniversary, the not-for-profit cell line repositories, CellBank Australia and JCRB Cell Bank, will disburse 40 Japan-derived cell line vials to Australia-based scientists, free-of-charge, for research into NCDs. With funding support from the Australia-Japan Foundation (AJF; Canberra, AU), the initiative will strengthen Australia's capacity for high-quality NCD research, an objective of the Global Action Plan for the Prevention and Control of NCDs, and will grow the literature related to the experimental use of the cell lines.
Click here to browse the CellBank Australia catalog of JCRB Cell Bank cell lines. Click here to lodge a request form for cell line(s) under this AJF-funded initiative. Click here to download a printable color flyer for your tearoom noticeboard.