Description: The cells possess a genomic amplification of the N-myc gene and express elevated levels of N-myc RNA or protein.

Also Known As:

Species: Human

Tissue: Brain

Growth Properties: Adherent

Morphology: Neuronal

Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5×1,000-2×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: No

Depositor:

Originator: No

References: Schwab M, Alitalo K, Klempnauer KH, Varmus HE, Bishop JM, Gilbert F, Brodeur G, Goldstein M, Trent J 1983 Amplified DNA with limited homology to myc cellular oncogene is shared by human neuroblastoma cell lines and a neuroblastoma tumour. Nature.; 305(5931):245-8. PMID: 6888561.

Hyperlink to ECACC Cell Line Data Sheet: 92110411