Description: KYSE-30 was established from the oesophageal cancer of an untreated 64 year old male. The tumour sample was taken from the mucosal surface of a well differentiated squamous cell carcinoma. The cell line KYSE-30 was established with the use of tumours initially transplanted to athymic mice. The cells are reported to have a doubling time of 20.8 hrs in the exponential growth phase. A p53 mutation at the splice acceptor site of intron 6 and a 12 fold amplification of c-erb B has been reported. KYSE-30 cells express a large number of epidermal growth factor receptors, 1.2×10,0,000 sites/cell.

Also Known As:

Species: Human

Tissue: oesophagus

Growth Properties: Adherent

Morphology: Polygonal

Growth Medium: RPMI 1640 + Ham’s F12 (1:1) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 1×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: No

Depositor: Dr Y Shimada, First Department of Surgery, Faculty of Medicine, Kyoto University, Kyoto

Originator: Yes

References: Shimada Y, Imamura M, Wagata T, Yamaguchi N, Tobe T.1992 Characterization of 21 newly established esophageal cancer cell lines. Cancer. 69(2):277-84. Erratum in: Cancer 1992 70(1):206 PMID: 1728357.

Hyperlink to ECACC Cell Line Data Sheet: 94072011