L14
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Description
Description: One of a series of cell lines established from mononuclear cells of a boar (Sus scrofa domestica L) which was injected with Shimozuma cells producing porcine retrovirus (Tsukuba-1). Contain retrovirus particles; positive in RT assay. Membrane contains u isotype molecules.
Also Known As:
Species: Porcine
Tissue: Blood
Growth Properties: Suspension
Morphology:
Growth Medium: RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Maintain cultures between 100,000 to 1,0,000 cells/ml; 5% CO2; 37°C. Cells grow as small clumps.
Release Conditions: No
Depositor: Dr B Kaeffer, INRA, Laboratoire Pathologie, Nouzilly
Originator: Yes
References: Kaeffer B, Bottreau E, Phan Thanh L, Olivier M, Salmon H.1990 Histocompatible miniature, boar model: selection of transformed cell lines of B and T lineages producing retrovirus. Int J Cancer. 46(3):481-8. PMID: 2394515
Hyperlink to ECACC Cell Line Data Sheet: 91012317
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