Description: Established from an African Green monkey foetal kidney. The cells are highly susceptible to Simian rotavirus SA11. Please note: The species of origin of the MA104 cell line has been shown to be different from that claimed by the originators. Whitaker & Hayward (1985) found the cell line to originate from an African Green monkey and not a Rhesus macaque as was originally claimed. Whitaker AM & Hayward CJ (1985) The characterization of three monkey kidney cell lines. Develop. Biol. Standard. Vol 60. Pp 125 to 131. PMID: 4043530.Abstract: Three monkey kidney cell lines, Vero, GL-V3 and MA-104 were subjected to karyological analysis to determine their chromosomal stability and to confirm their species of origin. Although the lines were shown to be relatively stable throughout all of the passage levels that were tested, the species of origin of one of them was found to be different from that claimed by the originators. This finding was supported by data from isoenzyme studies.

Also Known As:

Species: Monkey

Tissue: Kidney

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: Yes – CITES (Convention on the International Trade in Endangered Species of Wild Fauna and Flora) licence required for all orders outside the European Union

Depositor: Dr A Collins, AFRC Institute for Research on Animal Diseases, Compton

Originator: Yes

References: J Gen Virol 1979;43:513; Arch Virol 1981;70:33; Whitaker AM & Hayward CJ (1985) The characterization of three monkey kidney cell lines.  Develop. Biol. Standard. Vol 60. Pp 125 ? 131.  PMID: 4043530. 

Hyperlink to ECACC Cell Line Data Sheet: 85102918