MDCK-SIAT1
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Description
Description: This cell line has been derived by the stable transfection of MDCK cells with the cDNA of human 2,6-sialtransferase (SIAT1). The cells express two fold higher amounts of 6-linked sialic acids and two fold-lower amounts of 3-linked sialic acids than parent MDCK cells. MDCK-SIAT1 cells are presented as a suitable system for testing the sensitivity of human influenza virus to neuraminidase inhibitors (NAI) due to their over expression of sialyl-alpha2,6-galactose moieties.
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Species: Dog
Tissue: Kidney
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 1mg/ml G418 + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (85-95 %) 1:10 to 1:20 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin/EDTA; 37°C; 5% CO2.
Release Conditions: No
Depositor: Mikhail Matrosovich, Institute of Virology, Philipps University, Marburg, Germany. E-mail: Mikhail.Matrosovich@med.uni-marburg.de
Originator: Yes
References: Matrosovich M, Matrosovich T, Carr J, Roberts NA & Klenk H. (2003) Overexpression of the alpha-2, 6-sialyltransferase in MDCK cells increases influenza virus sensitivity to neuraminidase inhibitors. J. Virol. 77:8418-8425
Hyperlink to ECACC Cell Line Data Sheet: 05071502
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